Composite

Part:BBa_K4322002:Design

Designed by: Abraham Sinfort   Group: iGEM22_Cornell   (2022-09-27)


csgAα-asPink fusion protein with glycine-serine linker


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1388
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 1388
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1388
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1388
    Illegal NgoMIV site found at 58
    Illegal NgoMIV site found at 1193
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We optimized this sequence to be expressed in E. coli as part of our greater biobrick.


Source

The source of this part is the Escherichia coli strain K12.

References